Chk1 ps345
WebNov 4, 2024 · (G) Quantification of Sirius Red-stained area using at least 4 images per mouse (n = 5 per genotype). (H) Immunoblot of lysates from P14 isolated hepatocytes … WebCHK1 p-S345 Assay Kit CHK1 p-S345 Assay Kit PRODUCT NUMBER: TGRCHK1S10K With the exception of Control Lysates, this product is stable for at least 12 months from …
Chk1 ps345
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WebApr 5, 2024 · Relative levels of CHK1-pS345, RPA-pS33, and RPA-pS4/8 in CPT-treated cells are quantitated in Fig. S5c. (b) Cells were immunostained with α-RPA32-pS4/8 (red) and α-RPA32 (green) antibodies ... Web[pS345] - Chk1: Purity % Peak Area By HPLC ≥ 95%: Molecular Weight : 1147.2: Storage-20°C: Sequence (One-Letter Code) QGISF-pS-QPTC: Sequence (Three-Letter Code) H …
WebSep 23, 2024 · A total of 237 proteins were identified from 325 cases of LUSCC patients based on the TCPA and TCGA database. According to Kaplan-Meier survival analysis, univariate and multivariate Cox analysis, a prognostic prediction model was established which was consisted of 6 proteins (CHK1_pS345, CHK2, IRS1, PAXILLIN, BRCA2 and … WebJun 1, 2011 · In contrast, in normal tissues pS345 Chk1 appears to be a fairly rapid and short-lived response that is sensitive to the gemcitabine and AZD7762 doses. These findings suggest that pS345 Chk1 is a much more robust response in tumor than in normal tissue, which is consistent with the selective toxicity against tumors observed in our …
WebJul 6, 2024 · (C) Imaging-based quantification of pS345-CHK1-positive cells in DNA-PK-deficient (clone #2) and DNA-PK-proficient (control) MDA-MB-231 cells treated as indicated, as well as quantification of pT2609-DNA-PK and pS2056-DNA-PK in control cells. (E) Imaging-based quantification of γH2AX-positive cells in the replicating (EdU+) fraction. WebFeb 18, 2024 · Accordingly, depletion of DDX11 causes reduced levels of single-stranded DNA, a reduction of chromatin-bound replication protein A, and impaired CHK1 phosphorylation at serine-345. Taken together, we propose that DDX11 plays a role in dismantling secondary structures during DNA replication, thereby promoting CHK1 …
WebJan 14, 2024 · The samples were subjected to immunoblotting analysis of Chk1 pS345 and ATM pS1981. (C) HCT116 or (D) HeLa cells were transfected with control, MBNL2, p21 siRNA, respectively, and treated with 1 μM CPT for 4 h or mock-treated. Samples were collected and subjected to immunoblotting analysis of Chk1 pS345.
WebSpecific for Chk1 (phospho S345) in ELISA, with <1% reactivity with the non-modified form of the immunogen. Tested applications Suitable for: WB, IHC-P, ELISA more details … csc wifi loginWebAug 15, 2024 · Generation of CHK1-pS345 requires the presence of p53 protein in addition to the well-known activities of ATM/ATR kinases. Apparently, mutant forms of p53 possess higher activity in triggering CHK1 phosphorylation than wild type, implying a potential role of p53 in maintaining rDNA integrity. Loss of p53 function would cause replication stress ... csc win32iconWebThe AlphaLISA® SureFire® Ultra™ p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 … csc wimereuxWebGeneration of CHK1-pS345 requires the presence of p53 protein in addition to the well-known activities of ATM/ATR kinases. Apparently, mutant forms of p53 possess higher … csc wilmington addressWebJul 3, 2024 · The nice colocalization of Chk1-pS345, RPA32-pS4S8, and BLM foci with PML suggests that depletion of FANCM in ALT cells induces replication stress likely at the telomeres. To test this hypothesis, we … csc wilkes barreWeb[pS345]-Chk1 - This is amino acids 340 to 349 fragment of Chk-1 protein, phosphorylated at Ser345. Chk1, an evolutionarily conserved protein kinase, has been implicated in cell … csc win32resWebMar 21, 2024 · CHEK1 (Checkpoint Kinase 1) is a Protein Coding gene. Diseases associated with CHEK1 include Ataxia-Telangiectasia and Li-Fraumeni Syndrome . … dyson fan and heater review