WebCytbX was expressed and purified essentially as described previously ( 16, 27 ). Expression cultures were 1L LB medium in 2.5 L baffled flasks (Tunair). Cultures were inoculated from an overnight starter at 1:100 dilution and grown at 37 °C, 250 rpm, to an optical density at 600 nm (OD600) of 0.9. WebCytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). £164.00 - £7181.50 Specifications View More Specs Products 4 Description Specifications Description Prepacked with Ni Sepharose™ 6 Fast Flow media

HisTrap FF crude, 1 ml and 5 ml - Cytiva

WebHisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications. Packaging 1 pkg Features and Benefits WebHisTrap™ HP is a ready to use HiTrap™ column, prepacked with precharged Ni Sepharose™ High Performance . This prepacked column is ideal for preparative … how do i report eidl loan on taxes

Ni Sepharose 6 Fast Flow - Cytiva

WebPurification protocol for the protein purification procedure is found in the instructions for the selected column/media or at cytiva.com. 5 Ordering information Product Pack size Code No. His Buffer Kit 1 11-0034-00 His GraviTrap kit11 28-4013-51 His SpinTrap kit21 28-9321-71 1Includes 2 packs His GraviTrap and 1 pack His Buffer Kit Webcytiva.com71714900 AS HiTrapSP HP, 1 mL and 5 mL HiTrapQ HP, 1 mL and 5 mL Prepacked columns Instructions for Use HiTrap™ SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. how much money for passport in india