Fastgene gel/pcr extraction 300
WebDec 16, 2015 · The DNA was subjected to PCR and 300 ng of the PCR products were subjected to agarose gel electrophoresis ... The PCR amplicons were purified using a FastGene Gel/PCR extraction kit (NIPPON ... WebUp to 90% of DNA recovery from gel slices. PCR fragments of 300 bp were purified from 40 µl of a PCR stock solution using FastGene Gel/PCR Extraction Kit and a competitor kit, according to manufacturers protocol. …
Fastgene gel/pcr extraction 300
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WebSep 6, 2024 · The PCR products were purified using the FastGene ® Gel/PCR Extraction kit according to the manufacturer’s ... (Illumina) using the MiSeq Reagent Kit v3 (300 bp × 2 cycles with pair-end ... WebMaximum Sample Volume : 300 mg agarose gel or 100 µl PCR mix for a typical Recovery of 70 to 90%. Binding Capacity : 10 µg. DNA Fragment Length : 50 pb to 10 kb. Elution …
http://www.favorgen.com/favorgen/serv_1/mem_t1/h_1/pdf/fragment/FAGCK%20000%20001%20001-1.pdf WebFastGene® Gel/PCR Extraction Kit For extraction of DNA from agarose gels and purification of PCR products Cat.No.: FG-91202, 100 preparations ... Maximum Sample Volume 300 mg gel slice 100 µl PCR solution Gel < 2,5% TAE or TBE Typical Recovery 70 -80% 80 -90% Binding Capacity 10 µg 10 µg DNA Fragment Length 50 bp – 10 kbp 50 …
WebJun 22, 2024 · PCR products from the previous reactions were purified from an agarose gel using a FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan) and used as templates for mass amplification using TB71/CI78. sgRNA was synthesized and purified using the same kit. The concentration of the DNA template and the sgRNA were …
WebJan 9, 2024 · The RT-qPCR was carried out using the FastGene IC Green 2× qPCR mix (NIPPON Genetics, Tokyo, Japan), according to the manufacturer’s instructions, and the qTower 2.2 real-time PCR thermocycler (Analytik, Jena, Germany). Gene-specific primers were designed using the software Primer3 (; Table S11). All experiments were repeated …
WebJul 25, 2024 · For RNA extraction, a FastGene RNA Premium/Basic Kit (NIPPON Genetics Co. Ltd., Tokyo, Japan) was used according to the manufacturer’s instructions. cDNA was synthesized using PrimeScript™ Reverse Transcriptase (TaKaRa Bio Inc., Shiga, Japan) as directed by the manufacturer. PCR. The DNA and cDNA samples were used as … indian hawthorne shrubs near meWebFastGeneTM ゲル／PCR ... Gel/PCR Extraction kit FG-91202 100preps FG-91302 300preps Quick Guide DNA extraction from agarose gel / Purification of PCR products … indian hawthorne shrubs texasWebfor 30 s, and a final extension at 72 °C for 5 min. The PCR products were then purified by electrophoretic separation on a 2.0 % agarose gel with a FastGene Gel/PCR Extraction Kit (NIPPON Genetics Co., Ltd., Tokyo, Japan). The purifiedamplicons were pair-endsequenced (2× 300) on an Illumina MiSeq platform at the Hokkaido System local time in rabat