Furin is a protease, a proteolytic enzyme that in humans and other animals is encoded by the FURIN gene. Some proteins are inactive when they are first synthesized, and must have sections removed in order to become active. Furin cleaves these sections and activates the proteins. It was named furin … See more The protein encoded by this gene is an enzyme that belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their … See more Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 See more • Overview of all the structural information available in the PDB for UniProt: P09958 (Human Furin) at the PDBe-KB. • Overview of all the structural information available in the See more Furin has been shown to interact with PACS1. See more • Nakayama K (November 1997). "Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins" See more

Protocol - Cell Surface Flow Cytometry Staining Protocol

WebDefinition of furin in the Definitions.net dictionary. Meaning of furin. What does furin mean? Information and translations of furin in the most comprehensive dictionary definitions … WebIncubate on ice for 5 minutes. Stop cell lysis by adding 10ml Cell Staining Buffer to the tube. Centrifuge for 5 minutes at 350xg and discard supernatant. Repeat wash as in step 2. Count viable cells and resuspend in Cell Staining Buffer at 5-10 x 10 6 cells/ml and distribute 100µl/tube of cell suspension (5-10 x 10 5 cells/tube) into 12 x ... current time in gypsum colorado

National Center for Biotechnology Information

WebA truncated furin containing eluate from the Capto-MMC™ chromatography step was dialyzed against 50 mM Hepes buffer, 1 mM CaCl 2, pH 7,0 and approximately 33,000 U furin/ mL gel were loaded onto an Arginine-Sepharose 4B™ column (GE Healthcare) equilibrated in the same buffer. Truncated furin was eluted with a 0 - 250 mM NaCl … WebIn vivo active furin-overexpression-tumor-targeted imaging with FPR-675. Spectrally unmixed in vivo fluorescence images of tumor-bearing nude mouse at 6 h, 24 h, 48 h, and 72 h after intravenous injection of 100 μL FPR-675 (15 nmol) in PBS. LoVo tumor and MDA-MB-468 tumors are indicated with “L” and “M” by white arrows, respectively. WebFeb 1, 2011 · The amount of furin (fmol) per 10 6 cells was then calculated (based on lysing cells in 100 μl lysis buffer/10 6 cells). a [Furin] interpolated was below the reported MD … current time in gujarat india