WebDec 2, 2024 · Plant transgene-derived artificial small RNA molecules (sRNAs) can induce gene silencing in some insect pests, nematodes, fungi, and oomycetes, a phenomenon called “host-induced gene silencing” (HIGS; Jahan et al. 2015; Nowara et al. 2010). However, the limitation associated with HIGS is the requirement for the generation of transgenic … WebAug 18, 2024 · Host-induced gene silencing. Host-induced gene silencing is a plant transgene-mediated technique, in which plants express an RNAi construct designed against a specific gene(s) endogenous to the pathogen (Santala and Valkonen, 2024). The transgenes typically produce dsRNA or a hairpin-structured dsRNA construct, with …
Frontiers Host Induced Gene Silencing Targeting …
WebMar 28, 2024 · Host-induced gene silencing (HIGS) technology has emerged as a powerful alternative to chemical treatments for protecting plants from pathogens or pests. More than 170 HIGS studies have been published so far, and HIGS products have been launched. … WebRNA interference (RNAi) is a naturally occurring mechanism for gene silencing induced by the presence of short interfering RNA (siRNA). RNAi is an endogenous catalytic pathway that is triggered by double-stranded RNA (dsRNA). The trigger can occur naturally, as in the case of a cellular infection by a dsRNA virus, or by the intentional ... em munich livestream
Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop ...
WebJun 6, 2024 · Abstract. Host-induced gene silencing (HIGS) refers to the silencing of genes in pathogens and pests by expressing homologous double-stranded RNAs (dsRNA) or artificial microRNAs (amiRNAs) in the host plant. The discovery of such trans-kingdom RNA silencing has enabled the development of RNA interference-based approaches for … WebDec 1, 2024 · In this study, we used Host Induced Gene Silencing (HIGS) in an attempt to control white mold. Specific vectors were constructed to express a silencing hairpin (dsRNAs) of the pathogen effector genes Ss-caF1 (putative Ca 2+ binding protein), … WebIn addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more ... drain hose lowes