WebJun 20, 2000 · An important unresolved issue in the activation of the ISGF3 complex by IFN treatment concerns the location and dynamics of complex assembly and the signals used … WebThis complex, which was specific (i.e., blocked by excess unlabeled ISRE), corresponded to a polypeptide of -45 kDa after adjustment for crosslinked DNA (data not shown). We have shown (12) that ISGF3 can be formed upon mixing the cytoplasm of IFNc-treated cells (containing small amounts of ISGF3) and the cytoplasm of IFNy-treated cells

Regulation of type I interferon responses Nature Reviews

WebAug 11, 2009 · Autocrine or paracrine signaling by IFNβ triggers the JAK/STAT pathway and the timely up-regulation of many IFN stimulated genes (ISGs) through the phosphorylation of signal transducers and activators of transcription 1 (STAT1) and STAT2, and the assembly of the Interferon Stimulated Gene Factor 3 (ISGF3) complex composed of STAT1, STAT2, … WebMay 28, 2024 · In 1989, Levy et al. provided evidence that the active ISGF3 complex is already detectable within 2 min after exposure of cells to IFNα . Thus, the rapid ISGF3 assembly serves fast and robust IFNα responses that are diminished in time and coincide with the phosphorylation profiles of STAT1 and STAT2 . In this process, important … english long faced tumbler pigeons

Frontiers Fine-Tuning of Type I Interferon Response by STAT3

WebDec 23, 2013 · The interferon-stimulated gene factor 3 (ISGF3) complex (which is composed of STAT1, STAT2 and IFN-regulatory factor 9 (IRF9)) binds to IFN-stimulated response element (ISRE) sequences to activate ... WebThe first STAT proteins were identified as the regulatory cytoplasmic components of the IFN-γ-induced transcription factor complex, interferon-stimulated response element-3 … WebAbstract. Interferon-stimulated gene factor-3 (ISGF-3) is a multiprotein (113, 91, 84, and 48 kDa) transcriptional factor which regulates the expression of a specific set of genes, the … english major history minor