WebphiC31 integrase: Chromosome 4 (102F4), vasa promoter, marked with GFP and RFP Chromosome 2 attP Landing Site vas-int; attP40 (Stock 13-20) Genotype: y w M (eGFP, … Web湖北省农业科学院是省政府直属事业单位，是种植业、畜牧业、特产业相结合的综合性农业研究机构，是全省农业科学的最高学术机构和研究中心。 其主要职责是： (一)组织院属各研究单位开展农业科学的应用研究和应用基础研究，提高我省的农业科学技术水平。 (二)组织院属各单位对我省农业资源、生态资源等进行综合性考察、研究、试点，积累科学资料，为国 …
phiC31-integrase-mediated, site-specific integration of transgenes …
Web结果表明插入内含子序列的重组酶基因phiC31可在烟草植物中正确剪切表达重组酶,并精确诱导同向排列的attB与attP位点之间“封阻基因”Bar-E9序列删除,使得重组前处于“关闭”状态 … Web（phiC31やR4）によるヒト培養細胞への遺伝子導入実験 においても得られている9，10）．また，大腸菌ゲノム上へ挿 入されたattP 部位の中でも，複製起点oriC 近傍に … roadshow nrw
A Gateway MultiSite Recombination Cloning Toolkit
WebThe sterile insect technique (SIT) be an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae). Ionizing radiation employed to generates reproductive sterility in the mass-reared populations before release leads to weight of competitiveness. Here, we present … WebThe generation of DNA constructs belongs commonly adenine rate-limiting walk in conducting biological experiments. Recombination cloning the single DNA fragments using the Gateway method provided einem advance over established restriction enzyme cloning due the boosts in efficiency and reliability. Here were introduce adenine serial of entry … WebAbstract. Over the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. … roadshow modi