Phospho eif2a
WebHuman Phospho-eIF2 alpha (S52) Antibody Summary Species Reactivity Human Specificity Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Source Monoclonal Rat IgG 2B Clone # 849159 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human eIF2 alpha S52 site
Phospho eif2a
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WebJun 1, 2005 · Phospho-eIF2α restricts the activity of the guanine nuclear exchange factor eIF2B, which is responsible for the formation of the GTP–eIF2–tRNAi Met ternary complex. Because translation of most cellular and viral mRNA proceeds via ternary complex-dependent mechanisms, this leads to a global decrease in protein synthesis. WebFeb 26, 2015 · Phospho-eIF2α resistance has been observed both in yeast and in mammalian cells. In yeast, mutations in eIF2B (the GEF for eIF2) and eIF5 (the 48S PIC …
WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. WebFeb 10, 2015 · that mTOR signaling does influence phospho-eIF2a. For instance, the mTOR inhibitor rapamycin is known to upregulate phospho-eIF2a in some cells (Anand and Gruppuso, 2006; Kato et al., 2012; Kubota et al., 2003; Matsuo et al., 2005). Simi-larly, heat inactivation of mTOR potentiates phospho-eIF2a in
WebIn response to different environmental stresses, eIF2α phosphorylation represses global translation coincident with preferential translation of ATF4, a master regulator controlling the transcription of key genes essential for adaptative functions. WebAnti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications Suitable for: WB, IHC-P, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF or IP Species …
WebRayBio® Phospho-eIF-2A (S52) and Total eIF-2A ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated eIF-2A protein in your experimental model system, you can verify pathway activation in your cell lysates.
WebThe ratio of phospho-eIF2α to total eIF2α was determined by scanning densitometry and image analysis (Image J, NCBI). ... It is possible that queuine reduces the phosphorylation level of eIF2a ... can i take cyclobenzaprine at nightWebThe PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit #7286 detects endogenous levels of eIF2α protein when phosphorylated at Ser51 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. five more minutes scotty mccreery youtubeWebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … five more minutes on hallmarkWebSep 9, 2010 · Various forms of stress induce pathways that converge on the phosphorylation of the alpha (α) subunit of eukaryotic translation initiation factor eIF2 at serine 51 (S51), a modification that results in a global inhibition of protein synthesis. ... can i take cyclobenzaprine with alcoholWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health … can i take cyclobenzaprine with methocarbamolWebWestern Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody. Immunocytochemistry/ Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using eIF2a (Phospho-Ser51) antibody. can i take cyclobenzaprine with levothyroxineWebMay 3, 2016 · Here, we investigated the role of the EIF2AK4-EIF2A/eIF2α-ATF4 pathway in the autophagic response to AIEC infection. We showed that infection of human intestinal epithelial T84 cells with the AIEC reference strain LF82 activated the EIF2AK4-EIF2A-ATF4 pathway, as evidenced by increased phospho-EIF2AK4, phospho-EIF2A and ATF4 levels. five more minutes jonas brothers